Antibody and Immunoassay Services, HKU |
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Polyclonal Antibody Development
We provide quality services for production and purification of polyclonal antibodies in New Zealand rabbits. Our standard immunization protocol is summarized below, which can be modified depending on the immunogenecity of different antigens. We also provide technical support for antigen preparation (expression and purification of recombinant proteins, design and conjugation of peptide antigens etc), purification of antibodies using the affinity chromatography, and antibody characterization etc.
Days |
Task |
Notes |
Day 1 |
1st Immunization |
100ug antigen per rabbit |
Day 21 |
2nd Immunization |
100ug antigen per rabbit |
Day 42 |
3rd Immunization |
100ug antigen per rabbit |
Day 49 |
Bleed test |
ELISA to evaluate titer
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Day 56 |
Bleed production |
At least 40ml serum per rabbit |
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